Stefan Ståhl

KTH

ssta@kth.se

Keywords

Affinity protein, Cancer, Medical Imaging, Neurodegenerative disorders, Protein engineering, Targeted therapy

Key publications

Mestre Borras, A., Dahlsson Leitao, C., Ståhl, S. and Löfblom, J. Generation of an anti-idiotypic affibody-based masking domain for conditional activation of EGFR-targeting. New Biotechnology (2022). In press

Dahlsson Leitao, C., Ståhl, S and Löfblom, J. Bacterial display for selection of affibody molecules. In: Stefan Zielonka & Simon Krah (Eds). Genotype Phenotype Coupling: Methods and Protocols, Second Edition, Springer Science & Business Media, New York (2022) In press.

Faresjö, R., Lindberg, H., Ståhl, S., Löfblom, J., Syvänen, S. and Sehlin, D. Transferrin receptor binding BBB-shuttle facilitates brain delivery of anti-Aβ-affibodies. Pharmaceut. Research 39: 1509-1521 doi.org/10.1007/s11095-022-03282-2 (2022).

Rinne, S., Yin, W., Mestre Borras, A., Abouzaed, A., Dahlsson Leitao, C., Vorobyeva, A., Löfblom, J., Ståhl, S., Orlova, A. and Gräslund, T. Targeting tumor cells overexpressing the human epidermal growth factor receptor 3 with potent drug conjugates based on affibody molecules. Biomedicines 10: 1293, doi.org/10.3390/ 10061293 (2022).

Persson, J., Puuvuori, E., Zhang, B., Velikyan, I., Åberg, O., Müller, M., Nygren, P.-Å., Ståhl, S., Korsgren, O., Eriksson, O. and Löfblom, J. Discovery, optimization and biodistribution of an affibody molecule for imaging of CD69. Sci. Reports 11: 19151, doi.org/10.1038/s41598-021-97694-6 (2021).

Meister, S.W., Hjelm, L., Dannemeyer, M., Tegel, H., Lindberg, H., Ståhl, S. and Löfblom, J. An affibody molecule is actively transported into the cerebrospinal fluid via binding to the transferrin receptor. Int. J. Molec. Sciences 21, 2999; doi:10.3390/ijms21082999 (2020).

SciLifeLab / Contact / Stefan Ståhl

Stefan Ståhl

The research focus within my group is the protein engineering of small affinity domains, denoted affibody molecules and sequestrins, for applications in medicine, such as medical imaging and drug development.
A class of small affinity proteins, denoted affibody molecules, has been developed at our department. Staphylococcal protein A has served as scaffold for construction of combinatorial libraries from which affibody binding proteins can be selected to desired target proteins. Technology platforms to allow the generation of these affibodies is part of the research focus, and host-vector-systems for surface display on food grade staphylococci, e.g. Staphylococcus carnosus, as well as E. coli, are being evaluated for protein library applications using fluorescence activated cell sorting (FACS) for library screening.

The therapeutic potential of affibody molecules and sequestrins is investigated in various settings, including tumor targeting for in vivo imaging and biotherapy applications. Cancer biomarkers such as EGFR, HER2 and HER3 have been used in the context of tumor targeting, and mono- bi- and multispecific formats are being investigated.

In addition, affibodies are being investigated and evaluated in the context of neurodegenerative disorders, i.e. Alzheimer’s and Parkinson’s disease and frontotemporal dementia (FTD).The aim is to generate tools to study the development of the diseases in various models, to generate tracers to allow diagnosis by means of medical imaging and also to generate candidates for proteins therapeutics for evaluation in preclinical models.

Contact

ssta@kth.se