Workshop for Cryo-electron Tomography
March 10 @ 08:00 – March 14 @ 17:00 CET
Location: UCEM facility (EM-building) and Chemical Biological Centre (KBC) building, Umeå University, Linnaeus väg 6, Umeå
Deadline for registration: 13 January 2025
Cryo-electron tomography (cryo-ET) is a powerful structural technique. Its power comes from its versatility to study biological systems in vitro or in situ, i.e. within cells or tissue. It has the ability to reach uncharted regions in biological systems at high resolution. Advancements in microscopes, cameras, and computation make it now possible to determine sub-10 Å 3-dimensional structures of molecules directly within cells.
This four-and-a-half-day workshop will cover cryo-ET workflows from sample preparation on EM grids to data processing and analysis. The workshop will be a mixture of lectures and practical sessions. Participants will get hands-on experience in sample preparation on EM grids (micropatterning), sample freezing (plunge freezing and high-pressure freezing), cryo-correlative light and electron microscopy (cryo-CLEM), cryo-focused ion beam milling (cryo-FIB), cryo-TEM tiltseries data collection, and data processing and analysis (MotionCor2, IMOD, Dynamo).
It is compulsory that applicants have previous cryo-EM experience either with single-particle or already with cryo-tomography.
This workshop is supported by SciLifeLab, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM).
Contact email: erin.schexnaydre@umu.se
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