CryoEM sample preparation and data collection course
August 23, 2024 @ 08:00 – 17:00 CEST
The purpose of the course is to prepare and train cryoEM facility users in sample preparation methods, introduce users to the image data acquisition workflow, expand knowledge about cryoEM methods among researchers and show that everyone can learn how to use cryoEM for structural biology.
Course description
The course is open for facility users or potential facility users, such as PhD students, postdocs, and researchers within the life sciences who are curious and will profit from cryoEM skills. Swedish and international course participants are welcome. To attend, the course participants should be familiar with electron microscopy and structural biology.
This course is supported by SciLifeLab, CryoNET and Umeå University and organized by the Umeå Centre for Electron Microscopy (UCEM).
Application deadline
Application should be submitted before November 1st, 2024
Course organizers
Sara Sandin
Tanvir Shaikh
Location
UCEM and Chemical Biological Centre (KBC) Building
Umeå University
Linnaeus väg 6
Teachers and course instructors
- Linda Sandblad
- Sara Sandin
- Tanvir Shaikh
- Lars-Anders Carlson
- Erin Schexnaydre
- Johan Unge
- Additional teachers t.b.a
Course content
This course will cover practical aspects of cryoEM sample preparation and data acquisition.
The following topics will be covered:
- Overview of cryoEM methods
- Microscope design and electron optics
- Sample preparation
- Data collection for single particle analysis (SPA)
- Introduction to SPA image processing
The course consists of lectures, cryo lab work, demonstrations and practical exercises for all participants, hands-on experiences of microscopy operation and discussions. A detailed schedule will be provided to accepted participants.
Course literature
Handouts and material online.
Entry requirements
Priority will be given to researchers involved in a project where the use of cryo-EM is needed, which are to be briefly described upon application.
The course is suitable for researchers in the life sciences with a master’s or PhD degree, including graduate students, postdocs and staff scientists.
A maximum of 18 participants will be admitted. Please provide a clear description of your research project and motivation for attending the course.
Examination
Participation in all parts of the course, lectures, lab, microscopy demonstrations and evaluation are mandatory. Upon completion, SciLifeLab and Umeå University will provide a course certificate and a recommendation for 1 ECTS.
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